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KMID : 0361020020450121146
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Korean Journal of Otolaryngology - Head and Neck Surgery
2002 Volume.45 No. 12 p.1146 ~ p.1151
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Age-related Changes of Doublecortin Positive Cells in the Olfactory Bulb of the Rat
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여승근/Seung Geun Yeo
심현준/윤상원/권순욱/박경훈/허영범/차창일/조중생/Hyun Joon Shim/Sang Won Yoon/Soon Uk Kwon/Kyung Hoon Park/Young Buhm Huh/Chang Il Cha/Joong Saeng Cho
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Abstract
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Background and Objectives: It is well-established that neurogenesis continues to occur during life in the restricted brain areas, such as the glomerular and granule cell layers of the olfactory bulb. Doublecortin is a protein required for
neuronal migration in the developing brian and olfactory bulb, and is expressed in postmitotic migrating and differentiating neurons during embryonic and postnatal development periods. We investigated age-related changes of doublecortin positive
cells
in the olfactory bulb of aged rat compared with new born rat. Materials and Method: Four months old (control group, n=7) and 24 months old (aged group, n=7) male Fischer 344 rats were used in this study. Olfactory bulb of the rats were cut
into
40 ㎛-thick coronal sections and immunostained. We counted the doublecortin positive cells and neurofibrils, and measured the optical density of doublecortin by layer. We compared the results between the aged group and the control group.
Results:
In the olfactory bulbs of aged group, we observed less doublecortin positive cells, neurofibrils and lower optical density than the control group. Doublecortin is expressed during life in migratory neurobolasts of the olfactory bulb of the rats.
This
expression is reduced in the aged group and the reduced degree is variable according to the layer. Conclusion: Age-related changes of the olfactory bulb are associated with the reduction of postnatal neurogenesis, especially during the
migration
and differentiation stages. This changes result in reduction of interneurons of the olfactory bulb, and may be responsible for the decreased olfactory function.
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